First strand cdna synthesis kit roche manual

Increase the temperature of first-strand reaction (up to 50°C). Use PCR primers closer to the 3´ terminus of the target cDNA. Too little first-strand product was used in PCR Use up to 10 µl of the first-strand reaction. GSP was used for first-strand synthesis Try another GSP or switch to oligo(dT). Make sure the GSP is the antisense sequence. Roche Diagnostics TRANSCRIPTOR 1ST STRD CDNA KIT. Manufacturer: Roche Diagnostics Transcriptor First Strand cDNA Synthesis Kit; Contains a recombinant reverse transcriptase for the robust transcription of RNA fragments up . Fig. 2. The iScript Select cDNA Synthesis Kit facilitates synthesis of cDNA longer than 8 kb. First-strand cDNA was produced from 1 µg total RNA using the iScript Select cDNA Synthesis Kit and the provided oligo(dT) primer mix. A 2 µl aliquot of the 20 µl cDNA .

Fig. 2. The iScript Select cDNA Synthesis Kit facilitates synthesis of cDNA longer than 8 kb. First-strand cDNA was produced from 1 µg total RNA using the iScript Select cDNA Synthesis Kit and the provided oligo(dT) primer mix. A 2 µl aliquot of the 20 µl cDNA synthesis reaction was subjected to 35 PCR. First Strand cDNA Synthesis Kit for RT-PCR (AMV) sufficient for 30 reactions (including 5 control reactions), kit of 1 (10 components), suitable for RT-PCR, hotstart: no, dNTPs included; find Roche MSDS, related peer-reviewed papers, technical documents, similar products more at Sigma-Aldrich. First-Strand cDNA Synthesis Using SuperScript™ II RT, Continued The cDNA can now be used as a template for amplification in PCR. However, amplification of some PCR targets (1 kb) may require the removal of RNA complementary to the cDNA. To remove RNA complementary to the cDNA, add 1 μL (2 units) of E. coli RNase H and incubate at 37°C for.

Protocol. 9. Introduction. 9. First-strand cDNA synthesis owned by Hoffmann-La Roche Inc. Use of the PCR process requires a license. Publication protocol. Yeast cells () in mid-log phase were collected for total RNA extraction using the MasterPure yeast RNA purification kit (Epicentre). Reaction Protocol. Incubate the complete reaction mix in a thermal cycler using the following protocol: Priming. 5 min at 25ºC. Reverse transcription.